Description

• General description: High purity Fmoc-protected amino acid for research and process production of peptides, with very low levels of dipeptide, free-amino acids and acetic acid impurities. Standard building block for introduction of proline amino-acid residues by Fmoc SPPS Associated Protocols and Technical ArticlesFmoc-amino acids for Peptide ProductionCleavage and Deprotection Protocols for Fmoc SPPS
• Application: Biphasic electrochemical peptide synthesis: This study highlights the application of Fmoc-Pro-OH in electrochemical peptide synthesis, where slight excesses of reagents and electricity were required, possibly due to steric hindrance (S Nagahara, Y Okada, Y Kitano, K Chiba - Chemical Science, 2021). Elucidation of the Mechanism of Endo-XaaC-terminal Peptide Impurity Formation in SPPS: Fmoc-Pro-OH′s role was analyzed in a study investigating impurity formation during solid-phase peptide synthesis, highlighting its distinctively low tendency to form impurities (Y Yang, L Hansen, A Baldi - Organic Process Research & Development, 2021). Polymer–peptide delivery platforms: This research utilized Fmoc-Pro-OH in the development of polymer-based DNA delivery systems, emphasizing the effect of oligopeptide orientation on delivery efficacy (SS Parelkar, R Letteri, D Chan-Seng - Biomacromolecules, 2014). Structure–Activity Relationship Study of N-Hydroxyphtalimide Derivatives: The study explored the use of Fmoc-Pro-OH in the synthesis of N-Hydroxyphtalimide derivatives for detecting amines during peptide synthesis (K Takamatsu, R Suzuki, A Matsunaga - The Journal of Organic Chemistry, 2023).
• Linkage: Replaces: 04-12-1031
• Analysis Note: Colour (visual): white to off whiteAppearance of substance (visual): powderColour index (0,5 M in DMF): ≤ 150 HazenIdentity (IR): passes testEnantiomeric purity: ≥ 99.8 % (a/a)Purity (HPLC): ≥ 99.0 % (a/a)Fmoc-ß-Ala-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-β-Ala-Pro-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Pro-Pro-OH (HPLC): ≤ 0.1 % (a/a)Assay free amino acid (GC): ≤ 0.2 %Purity (TLC(011A)): ≥ 98 %Solubility (25 mmole in 50 ml DMF): clearly solubleAssay (acidimetric): ≥ 94.0 %Water (K. F.): ≤ 6.0 %Ethyl acetate (HS-GC): ≤ 0.5 %Acetate (IC): ≤ 0.02 %To see the solvent systems used for TLC of Novabiochem® products please click here.
• Legal Information: Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany