Description

• General description: Quasi-orthogonally-protected Lys derivative. The Fmoc group can be removed selectively by treatment with piperidine; the Dde group is cleaved with 2% hydrazine in DMF [1]. When removing Dde in the presence of allyl-based protecting groups, allyl alcohol should be included in the deprotection solution to prevent reduction of the allyl group [2]. Also available Fmoc-Lys(Dde)-OH 852057. This derivative has been employed in Fmoc SPPS to facilitate the introduction of biotin to the side-chain of lysine [3]. Associated Protocols and Technical Articles Guide to Selection of Orthogonally-Protected Building Blocks for Fmoc SPPS Literature references [1] B. W. Bycroft, et al. (1993) J. Chem. Soc., Chem. Commun., 778. [2] B. Rohwedder, et al. (1998) Tetrahedron Lett., 39, 1175. [3] J. Mack, et al. (1999) Lett. Pept. Sci., 6, 135.
• Linkage: Replaces: 04-12-5201
• Analysis Note: Colour (visual): white to slight yellow to beigeAppearance of substance (visual): powderIdentity (IR): passes testEnantiomeric purity: ≥ 99.5 % (a/a)Purity (TLC(157B)): ≥ 97 %Purity (TLC(CMA2)): ≥ 97 %Assay (HPLC, area%): ≥ 96.0 % (a/a)Solubility (1 mmole in 2 ml DMF): clearly solubleAssay (acidimetric): ≥ 90.0 %Water (K. F.): ≤ 1.00 %To see the solvent systems used for TLC of Novabiochem® products please click here.
• Legal Information: Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany