Jagged 2 Monoclonal Antibody (HMJ2-1), PE, eBioscience™, Invitrogen™

Description

• Description: This HMJ2-1 monoclonal antibody reacts with mouse Jagged-2, one of five type I transmembrane proteins that serves as a Notch receptor ligand
• Upon binding the Notch receptor (e.g., Notch 1-4), Jagged-2 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase
• The second cleavage event releases an intracellular fragment whose biological function remains controversial
• Jagged-2 is expressed by thymic lymphoid and stromal cells, as well as macrophages and dendritic cells in the spleen
• Note: Staining of endogenous splenic dendritic (CD8^+CD11c^+) cells could only be consistently confirmed in cells isolated from C57BL/6, but not Balb/c, mice
• Other strains have not been tested
• The antiobdy has been reported to crossreact to rat and have blocking function
• Applications Reported: This HMJ2-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HMJ2-1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development
• Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions
• JAG2 is one of several ligands that activate Notch and related receptors.