CD105 (Endoglin) Monoclonal Antibody (SN6), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin
• CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages
• It is suggested that CD105 functions in adhesion and embryonic angiogenesis
• Applications Reported: This SN6 antibody has been reported to have functional activity
• Applications Tested: This SN6 antibody has been tested by flow cytometric analysis of U937 cell line
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
• It exists on an O- and N-glycosylated homodimer
• Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
• CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.