Smad2/3 Mouse, Unlabeled, Clone: 18, BD

Description

• The transforming growth factor β (TGFβ)/activin/BMP family of growth factors plays a diverse and important role in growth, development, and differentiation
• These growth factors act through their binding to heteromeric plasma membrane receptor protein kinases which, upon ligand binding, become activated and trigger an intracellular signaling cascade
• Specifically, receptor activation induces the translocation of a set of conserved proteins named Smads (Sma- and Mad-related proteins) to the nucleus, resulting in gene activation
• Smad2 is a ubiquitously expressed protein of 58kDa that is phosphorylated and translocated to the nucleus in response to TGFβ, but not BMP
• The overall response to TGFβ is growth inhibition
• The Smad2 gene is located in chromosome 18q21.1 which is often absent in several human cancers
• Furthermore, some missense mutations on the Smad2 gene were identified in colorectal carcinomas, suggesting Smad2 may function as a tumor suppressor in normal cells
• Immunofluorescence, Immunoprecipitation, Western Blotting