Zika Virus Envelope (Domain III) (strain Zika SPH2015) Recombinant Rabbit Monoclonal Antibody (033), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E/Envelope protein (Domain III)
• As a member of the Flaviviridae family, ZIKV has a single positive sense RNA genome, which is initially translated as a single polyprotein which is then cleaved post-transitionally into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5)(1)
• The glycosated NS1 forms a homodimer inside the cells and is necessary for viral replication and late in infection (2)
• NS1 is also secreted into the extracellular space as a hexameric lipoprotein particle, which assists the virus in immune evasion and pathogenesis by interacting with components from both innate and adaptive immune systems as well as other host factors (1)
• Reference 1) Song, H.; Qi, J.; Haywood, J.; Shi, Y.; Gao, G.F
• (2016)
• Nat
• Struct
• Mol
• Biol
• 23, 456-458
• 2) Suthar, M.S., Diamond, M.S
• and Gale, M
• Jr
• (2013)
• Nat
• Rev
• Microbiol
• 11, 115-128.