BioTracker 519 Green β-Gal Dye, MilliporeSigma™

Description

• β-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond
• β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal
• β-gal has also been used to measure cellular senescence (SA-β-gal)
• The BioTracker 543 Green β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells
• It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence
• Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements
• The dye can stain LacZ expressing HEK293 cells both before and after 3% paraformaldehyde fixation
• Absorbance: 497 nm Emission: 519 nm