Invitrogen™ Luria Broth Base (Miller's LB Broth Base), powder

Description

• Luria broth (LB) is a popular, rich medium used to cultivate bacteria and to perform coliphage plaque assays
• The original formulation published in 1951 by Guiseppe Bertani to study phage plaque formation and named Lysogeny broth
• It is also known as Lennox broth and Luria-Bertani broth
• Widely used LB medium formulations differ in salt concentration: Miller: 10 g/L NaCl Lennox: 5 g/L NaCl Luria: 0.5 g/L NaCl Luria Broth Base (Miller's formulation) is recommended for the general maintenance and growth of E
• coli as it allows for fast cell expansion and supports high cell density
• In cases where salt-sensitive antibiotics are being used for selection (such as Zeocin, tetracycline or chloramphenicol), Lennox formulation medium would be recommended
• Luria Broth Base formulation Formulation per liter: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 10 g NaCl
• To prepare 1 L of Miller-LB media, add 25 g of powder to 1 L distilled water and mix by vortexing
• Adjust the pH to 7.0 with 5 N NaOH and sterilize by autoclaving for 25 minutes at 121°C
• Find the media format that fits your needs We offer other ready-to-use media formulations, such as LB Broth (Cat
• No
• 10855001), Terrific Broth (Cat
• No
• A1374301) or S.O.C recovery medium (Cat
• No
• 15544034)
• Order Info Shipping Condition: Room Temperature